Aim: Bacterial infection is a significant factor in the complex process of delayed wound healing. The advent of antibiotic resistance in bacterial isolates led the creation of antimicrobial drugs from new resources. Formulating the hydrogel using carbon nanoparticles synthesized from the burnt ash of Piper longum against the multidrug resistance skin infection causing pathogens were the goal of the current study. Materials and Methods: The dried fruit of Piper longum were collected from local market, burnt and carbon nanoparticles were synthesized from the burnt ash. The synthesized nanopartiocles were characterized and the anti-bacterial activity as well as the MIC value was assessed. Results: The absorption peak spectrum, size and shape were identified by UV-Vis spectrophotometry, Scanning Electron Microscopy (SEM) and X-ray Diffraction (XRD). Additionally, the functional group present in the synthesized carbon nanoparticles was detected by FTIR analysis. The synthesized carbon nanoparticles showed bactericidal action against Multidrug resistance Escherichia coli, Staphylococcus aureus and K.pneumoniae. Minimum inhibitory concentration, red blood cell hemolysis, Time kill assay and skin irritation test were performed. Conclusion: The developed wound healing hydrogel demonstrated both significant wound healing and antibacterial action. As a result, the formulated hydrogel has the potential to be employed as a dressing for wounds
