In the cultivation of grapes in Uzbekistan, phytopathogenic micromycetes cause dangerous diseases and cause the death of most of the crop. Therefore, it is important to identify the micromycetes that spread the disease from grapes. The article covers research aimed at studying micromycetes that spread disease in grapes grown in Uzbekistan. This makes it possible to grow ecologically clean products in viticulture and identify dangerous diseases in advance. The aim of our study was to study the microbiome of the soil of the grape growing area, infected young vines, leaves and fruits of grapes and to identify the phytopathogenic micromycetes that spread the disease. As part of our research, samples of infected branches, leaves, and fruits of “Kishmish” and “Rizamat” grape varieties grown in Urgut and Altyarik districts of Uzbekistan were brought. Phytopathogenic micromycetes were isolated from the samples. Cleaned 10 times with 3% hydrogen peroxide, alcohol and distilled water was grown meat-peptone agar (MPA), potatodextrose agar (PDA), Oat agar (ОА), Chapek nutrient media. Micromycete isolates belonging to the genera Fusarium, Alternaria, Penicillium and Aspergillus were detected in the soils of grape growing and in samples taken from infected grapes.