Medicinal plants have played an important role in the pharmacology and medicine. Pomegranate (Punica granatum L) an ancient fruit, has been used to investigate its anti-carcinogenic effect on the hormonal, lipid profile and histology of testis in Diethylnitrosoamine (DEN) induced albino rats. Group I served as normal control. Group II and Group III were given low and high dose DEN for 40 days. Group IV and Group V were treated with low and high dose DEN + aqueous pomegranate peel extract. Group VI and Group VII were treated with low and high dose DEN + ethanolic pomegranate peel extract. Finally animals were sacrificed, blood collected and assayed for hormone and testis histology. The level of LH, FSH were significantly increased in all the DEN treated pomegranate supplemented groups. The level of prolactin was significantly increased in both the low and high dose DEN induced groups. A slight increase in the level of testosterone observed only in the low dose DEN supplemented group. Except for the high dose DEN treatment, the other groups showed a significant decrease in cortisol level. While cholesterol was significantly increased in both the supplemented groups. The level of triglyceride was significantly increased in all the treatment groups. The level of HDL was significantly reduced in both the DEN treated groups. Low and high dose DEN treated testis showed slight distruption and complete disorganization of basement membrane and seminiferous tubules. Supplementation with APPE and EPPE showed the preservation of the architecture of seminiferous tubule.