Day by day aquatic ecosystem develops as challenging environment for sustainability of aquatic flora and fauna, due to unstability created by continuously changing water quality. Deterioration of water quality by natural and anthropogenic activities was continued, since beginning of human development. Now days, water quality is continuously rendering throughout the world and becomes a serious threat resulting scarcity of valued freshwater utilized for variety of purposes. Panchganga river is considered as one of the important river in Maharashtra, India. Rapidly declining water quality of river Panchganga was monitored for the period of March 2011 to February 2013. During investigation, various physicochemical parameters at different monitoring sites were assessed and interpreted statistically. Average results along with seasonal alterations in the physicochemical parameters at different monitoring sites confirmed average water quality of river Panchganga. However, noted as continuously polluting river due to discharge of tremendous amount of pollutants from nearby industries along with domestic sewage.