Aim: This study aimed to determine the phytochemical constituents present in the various extracts of Pimenta dioica stem through the standard qualitative biochemical and GCMS analysis. Materials and Methods: The phytochemical analysis was carried out according to the standard procedures. The total phenol and tannin was estimated using Folin ciocalteau method. The profiling of phytoconstituents present in the ethanolic extract of Pimenta dioica was done by Gas Chromatography-Mass Spectrometric (GCMS) analytic technique (Agilent Technologies). Results: The Phytochemical screening of Pimenta dioica stem extract revealed existence of various phytochemicals. The total phenolic and tannin content of the ethanolic extract was higher than aqueous extract. GCMS profiling indicated the presence of 21 bioactive compounds including Eugenol, 7(1H)-Pteridinone, Phenol, 4-(2-propenyl), 8-Methylnonanoic acid, methyl ester and Benzeneacetonitrile. The eugenol was the key component. Conclusion: The phytochemical and GCMS profiling of Pimenta dioica stem extract revealed the presence of bioactive constituents with vital therapeutic properties. Hence, isolation of individual phytochemical compounds may lead to the discovery of a novel therapeutic drug.
