Isolation, Screening and Identification of Benzene Degrading Bacterium, Pseudomonas putida

Asian Journal of Biological and Life Sciences ,2012,1,1,24-28.
Published:May 2012
Type:Research Article
Author(s) affiliations:

Gangula Sai Ramalinga Reddy1*, Koona Subramanyam2, Rajasekhar Pinnamaneni2

1Department of Microbiology, Sri Sai Baba National Degree and PG College, Anantapur 515001, Andhra Pradesh, INIDA.

2Department of Biotechnology, Sri Sai Baba National Degree and PG College, Anantapur 515001, Andhra Pradesh, INDIA.


The BTEX (benzene, toluene, ethylbenzene, and xylene) compounds are hydrocarbon pollutants that are considered as significant threat to the health and environment. Among BTEX, benzene is of major concern, because it is soluble, mobile, toxic, carcinogenic and one of the most stable aromatic compounds especially in ground and surface waters. Microbial degradation of benzene in aerobic environment has been successful, however, it is poorly biodegraded in anaerobic conditions. Pseudomonas species are common in aerobic bioremediation of benzene. The objective of this study was to identify the Benzene degrading Pseudomonas putida. Dilution plating was used to quantify the numbers of Pseudomonas putida, and DNA sequence analysis was used to identify Pseudomonas putida species. Pseudomonas putida was identified basing on the microscopic observations and Biochemical characterization. It was ultimately confirmed based on DNA sequence (16S rRNA sequence) analysis and finally concluded by BLAST analysis by constructing a phylogenetic tree.